dc.contributor.author | Cuevas Ramos, Gabriel | spa |
dc.contributor.author | Arguelles, David | spa |
dc.contributor.author | Vidal, Luis | spa |
dc.contributor.author | Prades, Marta | spa |
dc.date.accessioned | 2019-07-01 00:00:00 | |
dc.date.accessioned | 2020-12-09T16:52:37Z | |
dc.date.available | 2019-07-01 00:00:00 | |
dc.date.available | 2020-12-09T16:52:37Z | |
dc.date.issued | 2019-07-01 | |
dc.identifier.uri | https://doi.org/10.17151/vetzo.2019.13.2.5 | |
dc.identifier.uri | https://repositorio.ucaldas.edu.co/handle/ucaldas/13651 | |
dc.description.abstract | Introducción: el plasma rico en plaquetas (PRP) es un producto autólogo derivado de la sangre con varias aplicaciones terapéuticas. Se ha demostrado que los factores de crecimiento plaquetario estimulan la curación y reducen el dolor. Su uso en la industria equina, particularmente para problemas ortopédicos como la osteoartritis o la tendinitis ha ido en aumento. Sin embargo, el PRP carece de reproducibilidad y a veces, de calidad, ya que no solo los factores individuales (raza, edad, sexo) y ambientales (hora del día, estado de hidratación), sino también los métodos de laboratorio pueden afectar su calidad final. Objetivos: nuestro objetivo fue desarrollar y normalizar un protocolo para la preparación de PRP, que será repetible para uso equino, a través de un kit desechable estéril con una centrífuga estándar, que minimiza las variantes de laboratorio. Métodos y resultados: se estableció el protocolo final que resultó en un PRP con una concentración de plaquetas muy alta (6,4±1,3 veces mayor) y recuentos bajos de glóbulos blancos (3,7±1,8 x106/ml). El PRP final tenía concentraciones de factor de crecimiento derivado de plaquetas con un promedio de 37 ng/ml. Conclusión: El protocolo es altamente repetible y simple de realizar, y el kit es adecuado para usar en condiciones de campo porque no permite el contacto entre la muestra y el aire ambiente a lo largo del proceso. | spa |
dc.description.abstract | Introduction: Platelet-rich plasma (PRP) is an autologous blood-derived product with several therapeutic applications. Platelet growth factors have been shown to stimulate healing and reduce pain. Its use in the equine industry, particularly for orthopedic problems such as osteoarthritis or tendonitis has been increasing. However, PRP lacks reproducibility and, sometimes, quality since not only individual (breed, age, gender) and environmental (time of the day, hydration status) factors, but also laboratory methods can affect its final quality. Aims: we aimed to develop and normalize a protocol for PRP preparation, which will be repeatable for equine use, through a sterile disposable kit with a standard centrifuge, which minimizes laboratory variants. Methods and results: The final protocol resulting in a PRP with very high platelet concentration (6.4 ± 1.3 fold increase) and low white blood cell counts (3.7 ± 1.8 x106/mL) was stablished. The usual fold increase reported is 2 to 3. The final PRP had concentrations of platelet-derived growth factor with an average of 37ng/ml. Conclusion: The protocol is highly repeatable and simple to perform and the kit is suitable to use under field conditions because it does not permit contact between the sample and ambient air along the process. | eng |
dc.format.mimetype | application/pdf | eng |
dc.language.iso | eng | eng |
dc.publisher | Universidad de Caldas | spa |
dc.rights | Derechos de autor 2019 David Arguelles | eng |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-sa/4.0 | eng |
dc.source | https://revistasojs.ucaldas.edu.co/index.php/vetzootec/article/view/347 | eng |
dc.subject | platelet-rich plasma | eng |
dc.subject | horses | eng |
dc.subject | new method | eng |
dc.subject | plasma rico en plaquetas | spa |
dc.subject | caballos | spa |
dc.subject | nuevo método | spa |
dc.title | Desarrollo de un protocolo estandarizado y repetible para obtener plasma altamente concentrado rico en plaquetas en caballos. | spa |
dc.type | Sección Research / investigación | spa |
dc.type | Artículo de revista | spa |
dc.type | Journal Article | eng |
dc.identifier.doi | 10.17151/vetzo.2019.13.2.5 | |
dc.identifier.eissn | 2011-5415 | |
dc.relation.citationendpage | 62 | |
dc.relation.citationissue | 2 | spa |
dc.relation.citationstartpage | 52 | |
dc.relation.citationvolume | 13 | spa |
dc.relation.ispartofjournal | Revista Veterinaria y Zootecnia (On Line) | spa |
dc.relation.references | Alvarez, M. E., Giraldo, C. E., & Carmona, J. U. (2010). Monitoring bacterial contamination in equine platelet concentrates obtained by the tube method in a clean laboratory environment under three different technical conditions. Equine Veterinary Journal, 42(1), 63–67. https://doi.org/10.2746/042516409X455221 PMID:20121916 | eng |
dc.relation.references | Andia, I., and Abate, M. (2014). Knee osteoarthritis: Hyaluronic acid, platelet-rich plasma or both in association? Expert Opinion on Biological Therapy, 14(5), 635–649. https://doi.org/10.1517/14712598.2014.889677 PMID:24533435 | eng |
dc.relation.references | Andia, I., and Maffulli, N. (2013). Platelet-rich plasma for managing pain and inflammation in osteoarthritis. Nature Reviews. Rheumatology, 9(12), 721–730. https://doi.org/10.1038/nrrheum.2013.141 PMID:24080861 | eng |
dc.relation.references | Anitua, E., Zalduendo, M. M., Alkhraisat, M. H. and Orive, G. (2013). Release kinetics of platelet-derived and plasma-derived growth factors from autologous plasma rich in growth factors. Annals of Anatomy, 195(5), 461–466. https://doi.org/10.1016/j.aanat.2013.04.004 PMID:23722041 | eng |
dc.relation.references | Argüelles, D., Carmona, J. U., Pastor, J., Iborra, A., Viñals, L., Martínez, P., Prades, M. (2006). Evaluation of single and double centrifugation tube methods for concentrating equine platelets. Research in Veterinary Science, 81(2), 237–245. https://doi.org/10.1016/j.rvsc.2005.12.008 | eng |
dc.relation.references | Carmona, J. U., and López, C. (2011). Autologous Platelet Concentrates as a Treatment for Shoulder Injury in a Horse. Journal of Equine Veterinary Science, 31(9), 506– 510. https://doi.org/10.1016/j.jevs.2011.03.008 | eng |
dc.relation.references | Cerciello, S., Beitzel, K., Howlett, N., Russell, R. P., Apostolakos, J., McCarthy, M. B., Mazzocca, A. D. (2013). The Use of Platelet-Rich Plasma Preparations in the Treatment of Musculoskeletal Injuries in Orthopaedic Sports Medicine. Operative Techniques in Orthopaedics, 23(2), 69–74. https://doi.org/10.1053/j.oto.2013.07.001 | eng |
dc.relation.references | Coppinger, J. A., Cagney, G., Toomey, S., Kislinger, T., Belton, O., McRedmond, J. P., Maguire, P. B. (2004). Characterization of the proteins released from activated platelets leads to localization of novel platelet proteins in human atherosclerotic lesions. Blood,103(6), 2096–2104. https://doi.org/10.1182/blood-2003-08-2804 PMID:14630798 | eng |
dc.relation.references | Dohan Ehrenfest, D. M., Bielecki, T., Mishra, A., Borzini, P., Inchingolo, F., Sammartino, G., Everts, P. A. (2012). In search of a consensus terminology in the field of platelet concentrates for surgical use: Platelet-rich plasma (PRP), platelet-rich fibrin (PRF), fibrin gel polymerization and leukocytes. Current Pharmaceutical Biotechnology, 13(7), 1131–1137. https://doi.org/10.2174/138920112800624328 PMID:21740379 | eng |
dc.relation.references | Dohan Ehrenfest, D. M., Rasmusson, L., and Albrektsson, T. (2009). Classification of platelet concentrates: From pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF). Trends in Biotechnology, 27(3), 158–167. https://doi.org/10.1016/j.tibtech.2008.11.009 | eng |
dc.relation.references | Fontenot RL, Sink CA, Werre SR, Weinstein NM, Dahlgren LA. Simple tube centrifugation for processing platelet-rich plasma in the horse. Can Vet J = La Rev Vet Can 2012;53:1266–72. | eng |
dc.relation.references | Fortier, L. A., Barker, J. U., Strauss, E. J., McCarrel, T. M., and Cole, B. J. (2011). The Role of Growth Factors in Cartilage Repair. Clinical Orthopaedics and Related Research, 469(10), 2706–2715. https://doi.org/10.1007/s11999-011-1857-3 | eng |
dc.relation.references | Giraldo, C. E., López, C., Álvarez, M. E., Samudio, I. J., Prades, M., and Carmona, J. U. (2013). Effects of the breed, sex and age on cellular content and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel. BMC Veterinary Research, 9(1), 29. https://doi.org/10.1186/1746-6148-9-29 | eng |
dc.relation.references | Graziani, F., Ivanovski, S., Cei, S., Ducci, F., Tonetti, M., and Gabriele, M. (2006). The in vitro effect of different PRP concentrations on osteoblasts and fibroblasts. Clinical Oral Implants Research, 17(2), 212–219. https://doi.org/10.1111/j.1600-0501.2005.01203.x PMID:16584418 | eng |
dc.relation.references | Holmes, H. L., Wilson, B., Goerger, J. P., Silverberg, J. L., Cohen, I., Zipfel, W. R., and Fortier, L. A. (2018). Facilitated recruitment of mesenchymal stromal cells by bone marrow concentrate and platelet rich plasma. PLoS One, 13(3), e0194567. https://doi.org/10.1371/journal.pone.0194567 | eng |
dc.relation.references | Kawasumi, M., Kitoh, H., Siwicka, K. A., and Ishiguro, N. (2008). The effect of the platelet concentration in platelet-rich plasma gel on the regeneration of bone. The Journal of Bone and Joint Surgery. British Volume, 90(7), 966–972. https://doi.org/10.1302/0301-620X.90B7.20235 PMID:18591611 | eng |
dc.relation.references | Li, A., Xia, X., Yeh, J., Kua, H., Liu, H., Mishina, Y., Li, B. (2014). PDGF-AA promotes osteogenic differentiation and migration of mesenchymal stem cell by downregulating PDGFRα and derepressing BMP-Smad1/5/8 signaling. PLoS One, 9(12), e113785. https://doi.org/10.1371/journal.pone.0113785 | eng |
dc.relation.references | Magalon, J., Bausset, O., Serratrice, N., Giraudo, L., Aboudou, H., Veran, J., Sabatier, F. (2014). Characterization and Comparison of 5 Platelet-Rich Plasma Preparations in a Single-Donor Model. Arthrosc J Arthrosc Relat Surg, 30(5), 629–638. https://doi.org/10.1016/j.arthro.2014.02.020 | eng |
dc.relation.references | Marx, R. E. (2004). Platelet-rich plasma: Evidence to support its use. Journal of Oral and Maxillofacial Surgery, 62(4), 489–496. https://doi.org/10.1016/j.joms.2003.12.003 PMID:15085519 | eng |
dc.relation.references | Marx, R. E., Carlson, E. R., Eichstaedt, R. M., Schimmele, S. R., Strauss, J. E., and Georgeff, K. R. (1998). Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, 85(6), 638–646. https://doi.org/10.1016/S1079-2104(98)90029-4 PMID:9638695 | eng |
dc.relation.references | McCarrel TM., Minas T., Fortier, LA. (2012). Optimization of leukocyte concentration in platelet-rich plasma for the treatment of tendinopathy. J Bone Joint Surg Am. 94:e143(1-8). doi:10.2106/JBJS.L.00019. | eng |
dc.relation.references | O’Shea, C. M., Werre, S. R., and Dahlgren, L. A. (2015). Comparison of platelet counting technologies in equine platelet concentrates. Veterinary Surgery, 44(3), 304–313. https://doi.org/10.1111/j.1532-950X.2014.12290.x PMID:25307726 | eng |
dc.relation.references | Pichereau, F., Décory, M., and Cuevas Ramos, G. (2014). Autologous platelet concentrate as a treatment for horses with refractory fetlock osteoarthritis. Journal of Equine Veterinary Science, 34(4), 489–493. https://doi.org/10.1016/j.jevs.2013.10.004 | eng |
dc.relation.references | Rinnovati, R., Romagnoli, N., Gentilini, F., Lambertini, C., and Spadari, A. (2015). The influence of environmental variables on platelet concentration in horse platelet-rich plasma. Acta Veterinaria Scandinavica, 58(1), 45. https://doi.org/10.1186/s13028-016-0226-3 | eng |
dc.relation.references | Satué, K., Gardón, J., and Muñoz, A. (2017). Interpretation of Platelets in The Horse. Journal of Hematology Research, 4, 19–25. https://doi.org/10.12974/2312-5411.2017.04.3 | eng |
dc.relation.references | Spaková, T., Rosocha, J., Lacko, M., Harvanová, D., and Gharaibeh, A. (2012). Treatment of knee joint osteoarthritis with autologous platelet-rich plasma in comparison with hyaluronic acid. American Journal of Physical Medicine & Rehabilitation, 91(5), 411–417. https://doi.org/10.1097/PHM.0b013e3182aab72 | eng |
dc.relation.references | Sundman, E. A., Cole, B. J., and Fortier, L. A. (2011). Growth factor and catabolic cytokine concentrations are influenced by the cellular composition of platelet-rich plasma. The American Journal of Sports Medicine, 39(10), 2135–2140. https://doi.org/10.1177/0363546511417792 PMID:21846925 | eng |
dc.relation.references | Sundman, E. A., Cole, B. J., Karas, V., Della Valle, C., Tetreault, M. W., Mohammed, H. O., and Fortier, L. A. (2014). The anti-inflammatory and matrix restorative mechanisms of platelet-rich plasma in osteoarthritis. The American Journal of Sports Medicine, 42(1), 35–41. https://doi.org/10.1177/0363546513507766 PMID:24192391 | eng |
dc.relation.references | Wasserman, A., Matthewson, G., and MacDonald, P. (2018). Platelet-Rich Plasma and the Knee-Applications in Orthopedic Surgery. Current Reviews in Musculoskeletal Medicine, 11(4), 607–615. https://doi.org/10.1007/s12178-018-9521-0 | eng |
dc.relation.references | Zhu, Y., Yuan, M., Meng, H. Y., Wang, A. Y., Guo, Q. Y., Wang, Y., and Peng, J. (2013). Basic science and clinical application of platelet-rich plasma for cartilage defects and osteoarthritis: A review. Osteoarthritis and Cartilage, 21(11), 1627–1637. https://doi.org/10.1016/j.joca.2013.07.017 PMID:23933379 | eng |
dc.rights.accessrights | info:eu-repo/semantics/openAccess | eng |
dc.rights.creativecommons | Esta obra está bajo licencia internacional Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0. | eng |
dc.title.translated | Development of a standardized and repeatable protocol to obtain highly concentrated platelet-rich plasma in horses. | eng |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | eng |
dc.type.content | Text | eng |
dc.type.driver | info:eu-repo/semantics/article | eng |
dc.type.version | info:eu-repo/semantics/publishedVersion | eng |
dc.relation.citationedition | Núm. 2 , Año 2019 : Julio - Diciembre | spa |
dc.relation.bitstream | https://revistasojs.ucaldas.edu.co/index.php/vetzootec/article/download/347/276 | |
dc.type.coarversion | http://purl.org/coar/version/c_970fb48d4fbd8a85 | eng |
dc.rights.coar | http://purl.org/coar/access_right/c_abf2 | eng |